H. Wang, H. Zhang, Y.H. Wong, C.R. Voolstra, T. Ravasi, V. Bajic, P.Y. Qian
Proteomics, volume 10, issue 16, pp. 2972-2981, no. 16, (2010)
Animal proteomics, Bugula neritina, Larval metamorphosis, Pyrosequencing, Transcriptome
Non-model organisms represent the majority of life forms in our planet.
However, the lack of genetic information hinders us to understand the
unique biological phenomena in non-model organisms at the molecular
level. In this study, we applied a tandem transcriptome and proteome
profiling on a non-model marine fouling organism, Bugula neritina.
Using a 454 pyrosequencing platform with the updated titanium reagents,
we generated a total of 48M bp transcriptome data consisting of 131 450
high-quality reads. Of these, 122 650 reads (93%) were assembled to
produce 6392 contigs with an average length of 538 bases and the
remaining 8800 reads were singletons. Of the total 15 192 unigenes,
13 863 ORFs were predicated, of which 6917 were functionally annotated
based on gene ontology and eukaryotic orthologous groups. Subsequent
proteome analysis identified and quantified 882 proteins from B. neritina.
These results would provide fundamental and important information for
the subsequent studies of molecular mechanism in larval biology,
development, antifouling research. Furthermore, we demonstrated, for the
first time, the combined use of two high-throughput technologies as a
powerful approach for accelerating the studies of non-model but
otherwise important species.