High-resolution phenotypic profiling of natural products-induced effects on the single-cell level

S. Kremb, C.R. Voolstra
Sci. Rep., 7, 44472, (2017)

High-resolution phenotypic profiling of natural products-induced effects on the single-cell level

Keywords

Natural products (NPs) , Cytological profiling

Abstract

​Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.​​​

Code

DOI: 10.1038/srep44472

Sources

Website PDF

See all publications 2017