Flow cytometry detection of planktonic cells with polycyclic aromatic hydrocarbons sorbed to cell surfaces

M.I. Cerezo, M. Linden and S. Agustí
Marine Pollution Bulletin, volume 118, issues 12, pp. 64-70, (2017)

Flow cytometry detection of planktonic cells with polycyclic aromatic hydrocarbons sorbed to cell surfaces

Keywords

PAHs, Flow cytometry, Phytoplankton, Oil spills, Cell fluorescence, Ultraviolet laser

Abstract

​Polycyclic aromatic hydrocarbons are very important components of oil pollution. These pollutants tend to sorb to cell surfaces, exerting toxic effects on organisms. Our study developed a flow cytometric method for the detection of PAHs sorbed to phytoplankton by exploiting their spectral characteristics. We discriminated between cells with PAHs from cells free of PAHs. Clear discrimination was observed with flow cytometer provided with 375 or 405 nm lasers in addition to the standard 488 nm laser necessary to identify phytoplankton. Using this method, we measured the relationship between the percentages of phytoplankton organisms with PAHs, with the decrease in the growth rate. Moreover, the development of this method could be extended to facilitate the study of PAHs impact on cell cultures from a large variety of organisms.

Code

http://doi.org/10.1016/j.marpolbul.2017.02.006

Sources

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